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CYB5R3 / Dia 1 Antibody (C-Term)

Peptide-affinity purified goat antibody

     
  • WB - CYB5R3 / Dia 1  Antibody (C-Term) AF3454a
    AF3454a (0.01 µg/ml) staining of Human Umbilical Cord lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - CYB5R3 / Dia 1  Antibody (C-Term) AF3454a
    AF3454a (3.8 µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P00387
Other Accession NP_000389.1, NP_001123291.1, NP_001165131.1, 1727
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 34235 Da
Additional Information
Gene ID 1727
Other Names NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, 1.6.2.2, Diaphorase-1, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, CYB5R3, DIA1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYB5R3 / Dia 1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYB5R3 (HGNC:2873)
Synonyms DIA1
Function Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
Cellular Location [Isoform 1]: Endoplasmic reticulum membrane; Lipid-anchor {ECO:0000250|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250|UniProtKB:P20070}. Mitochondrion outer membrane; Lipid-anchor {ECO:0000250|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250|UniProtKB:P20070}
Tissue Location [Isoform 2]: Expressed at late stages of erythroid maturation.
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize all three reported isoforms (NP_000389.1; NP_001123291.1; NP_001165131.1).

References

RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1. Fernandez-Borja M, Janssen L, Verwoerd D, Hordijk P, Neefjes J, J. Cell. Sci. 2005 Jun 118 (Pt 12): 2661-70. PMID: 15944396

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$ 341.00
Cat# AF3454a
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