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NDUFS1 Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - NDUFS1 Antibody (internal region) AF3264a
    AF3264a (0.3 µg/ml) staining of Human Heart lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
    detail
  • IHC - NDUFS1 Antibody (internal region) AF3264a
    AF3264a (5 µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P28331
Other Accession NP_004997.4, 4719, 227197 (mouse), 301458 (rat)
Reactivity Human, Mouse
Predicted Rat, Rabbit, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 79468 Da
Additional Information
Gene ID 4719
Other Names NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial, 1.6.5.3, 1.6.99.3, Complex I-75kD, CI-75kD, NDUFS1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUFS1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFS1
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:30879903, PubMed:31557978). Essential for catalysing the entry and efficient transfer of electrons within complex I (PubMed:31557978). Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes (PubMed:30879903, PubMed:31557978).
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:P15690}; Matrix side {ECO:0000250|UniProtKB:P15690}
Research Areas
Citations (0)
citation

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References

Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects. Saito A, Kawamoto M, Kamatani N, Journal of human genetics 2009 Jun 54 (6): 317-23. PMID: 19343046

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$ 341.00
Cat# AF3264a
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