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POU6F2 Antibody (N-Term)

Peptide-affinity purified goat antibody

     
  • WB - POU6F2 Antibody (N-Term) AF3206a
    AF3206a (0.3 µg/ml) staining of Mouse Eye lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P78424
Other Accession NP_009183.3, NP_001159490.1, 11281
Reactivity Mouse
Predicted Human, Rat
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 73265 Da
Additional Information
Gene ID 11281
Other Names POU domain, class 6, transcription factor 2, Retina-derived POU domain factor 1, RPF-1, POU6F2, RPF1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOU6F2 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POU6F2
Synonyms RPF1
Function Probable transcription factor likely to be involved in early steps in the differentiation of amacrine and ganglion cells. Recognizes and binds to the DNA sequence 5'-ATGCAAAT-3'. Isoform 1 does not bind DNA.
Cellular Location Nucleus.
Tissue Location Expressed only within the CNS, where its expression is restricted to the medical habenulla, to a dispersed population of neurons in the dorsal hypothalamus, and to subsets of ganglion and amacrine cells in the retina.
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize both reported isoforms (NP_009183.3; NP_001159490.1).

References

Germline mutations of the POU6F2 gene in Wilms tumors with loss of heterozygosity on chromosome 7p14. Perotti D, De Vecchi G, Testi MA, Lualdi E, Modena P, Mondini P, Ravagnani F, Collini P, Di Renzo F, Spreafico F, Terenziani M, Sozzi G, Fossati-Bellani F, Radice P, Human mutation 2004 Nov 24 (5): 400-7. PMID: 15459955

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$ 341.00
Cat# AF3206a
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Availability: 7-10 days
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