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> home > Products > Primary Antibodies > Antibody Collections > Goat Antibodies > HUWE1 / LASU1 Antibody (internal region)
HUWE1 / LASU1 Antibody (internal region)
Peptide-affinity purified goat antibody
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- CITATIONS
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- BACKGROUND
Application 
| E |
|---|---|
| Primary Accession | Q7Z6Z7 |
| Other Accession | NP_113584.3, 10075, 59026 (mouse) |
| Predicted | Human, Mouse |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/ml |
| Isotype | IgG |
| Calculated MW | 481891 Da |
| Gene ID | 10075 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase HUWE1, 6.3.2.-, ARF-binding protein 1, ARF-BP1, HECT, UBA and WWE domain-containing protein 1, Homologous to E6AP carboxyl terminus homologous protein 9, HectH9, Large structure of UREB1, LASU1, Mcl-1 ubiquitin ligase E3, Mule, Upstream regulatory element-binding protein 1, URE-B1, URE-binding protein 1, HUWE1, KIAA0312, KIAA1578, UREB1 |
| Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HUWE1 / LASU1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HUWE1 |
|---|---|
| Synonyms | KIAA0312, KIAA1578, UREB1 |
| Function | E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15567145, PubMed:15767685, PubMed:15989957, PubMed:17567951, PubMed:18488021, PubMed:19037095, PubMed:19713937, PubMed:20534529, PubMed:30217973). Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1 (PubMed:15989957). Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair (PubMed:19713937). Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4 (PubMed:15567145, PubMed:15767685, PubMed:15989956). Ubiquitinates MFN2 to negatively regulate mitochondrial fusion in response to decreased stearoylation of TFRC (PubMed:26214738). Ubiquitination of MFN2 also takes place following induction of mitophagy; AMBRA1 acts as a cofactor for HUWE1-mediated ubiquitination (PubMed:30217973). Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN (PubMed:18488021). May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation (PubMed:17567951). Mediates polyubiquitination of isoform 2 of PA2G4 (PubMed:19037095). Acts in concert with MYCBP2 to regulate the circadian clock gene expression by promoting the lithium-induced ubiquination and degradation of NR1D1 (PubMed:20534529). Binds to an upstream initiator-like sequence in the preprodynorphin gene (By similarity). Mediates HAPSTR1 degradation, but is also a required cofactor in the pathway by which HAPSTR1 governs stress signaling (PubMed:35776542). Acts as a regulator of the JNK and NF-kappa-B signaling pathways by mediating assembly of heterotypic 'Lys-63'-/'Lys- 48'-linked branched ubiquitin chains that are then recognized by TAB2: HUWE1 mediates branching of 'Lys-48'-linked chains of substrates initially modified with 'Lys-63'-linked conjugates by TRAF6 (PubMed:27746020). 'Lys-63'-/'Lys-48'-linked branched ubiquitin chains protect 'Lys-63'-linkages from CYLD deubiquitination (PubMed:27746020). Ubiquitinates PPARA in hepatocytes (By similarity). |
| Cellular Location | Cytoplasm. Nucleus. Mitochondrion. Note=Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Recruited to mitochondria following interaction with AMBRA1 (PubMed:30217973) {ECO:0000250|UniProtKB:Q7TMY8, ECO:0000269|PubMed:30217973} |
| Tissue Location | Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Regulation of heat-induced apoptosis by Mcl-1 degradation and its inhibition by Hsp70. Stankiewicz AR, Livingstone AM, Mohseni N, Mosser DD. Cell death and differentiation 2009 Apr 16 (4): 638-47. PMID: 19148187
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$ 255.00
Cat# AF3010a
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