MARCH-I Antibody (internal region)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| E |
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Primary Accession | Q8TCQ1 |
Other Accession | NP_060393.1, 55016, 72925 (mouse), 361135 (rat) |
Predicted | Human, Mouse, Rat |
Host | Goat |
Clonality | Polyclonal |
Concentration | 0.5 mg/ml |
Isotype | IgG |
Calculated MW | 32308 Da |
Gene ID | 55016 |
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Other Names | E3 ubiquitin-protein ligase MARCH1, 6.3.2.-, Membrane-associated RING finger protein 1, Membrane-associated RING-CH protein I, MARCH-I, RING finger protein 171, MARCH1, RNF171 |
Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MARCH-I Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MARCHF1 (HGNC:26077) |
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Synonyms | MARCH1, RNF171 |
Function | E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system. |
Cellular Location | Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:Q6NZQ8}; Multi-pass membrane protein. Lysosome membrane; Multi- pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein |
Tissue Location | Expressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung. Expression is high in follicular B-cells, moderate in dendritic cells and low in splenic T-cells. |
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Provided below are standard protocols that you may find useful for product applications.
References
Novel regulation of MHC class II function in B cells. Matsuki Y, Ohmura-Hoshino M, Goto E, Aoki M, Mito-Yoshida M, Uematsu M,
Hasegawa T, Koseki H, Ohara O, Nakayama M, Toyooka K, Matsuoka K, Hotta H,
Yamamoto A, Ishido S. EMBO J. 2007 Feb 7;26(3):846-54. PMID: 17255932
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