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CDKN2A (isoform 3) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - CDKN2A (isoform 3) Antibody (internal region) AF2493a
    EB07091 (1 µg/ml) staining of HeLa lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P42771
Other Accession NP_478104.1, 1029
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 16533 Da
Additional Information
Gene ID 1029
Other Names Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3, Cyclin-dependent kinase 4 inhibitor A, CDK4I, Multiple tumor suppressor 1, MTS-1, p16-INK4a, p16-INK4, p16INK4A, CDKN2A, CDKN2, MTS1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDKN2A (isoform 3) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDKN2A (HGNC:1787)
Synonyms CDKN2, MTS1
Function Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
Cellular Location Cytoplasm. Nucleus
Tissue Location Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize the pancreas isoform (NP_478104.1) only.

References

p14ARF induces G2 cell cycle arrest in p53- and p21-deficient cells by down-regulating p34cdc2 kinase activity. Normand G, Hemmati PG, Verdoodt B, von Haefen C, Wendt J, Guner D, May E, Dorken B, Daniel PT. J Biol Chem. 2005 Feb 25;280(8):7118-30. Epub 2004 Dec 6. PMID: 15582998

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$ 341.00
Cat# AF2493a
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