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Histamine Receptor H2 Antibody (C-Term)

Peptide-affinity purified goat antibody

     
  • WB - Histamine Receptor H2 Antibody (C-Term) AF2456a
    EB06905 (2µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
    detail
  • IF - Histamine Receptor H2 Antibody (C-Term) AF2456a
    EB06905 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
    detail
  • FC - Histamine Receptor H2 Antibody (C-Term) AF2456a
    EB06905 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC, IF, E
Primary Accession P25021
Other Accession NP_071640.1, NP_001124527.1, 3274
Reactivity Rat
Predicted Human, Mouse, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 40098 Da
Additional Information
Gene ID 3274
Other Names Histamine H2 receptor, H2R, HH2R, Gastric receptor I, HRH2
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHistamine Receptor H2 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HRH2
Function The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein.
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognise both reported isoforms.

References

Histamine: its novel role as an endogenous regulator of Con A-dependent T cell proliferation. Nakane H, Sonobe Y, Watanabe T, Nakano K. Inflamm Res. 2004 Jul;53(7):324-8. Epub 2004 Jun 25. PMID: 15241568

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$ 341.00
Cat# AF2456a
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