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CTDSP1 Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - CTDSP1 Antibody (internal region) AF2446a
    AF2446a (0.1 µg/ml) staining of Mouse Testis lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - CTDSP1 Antibody (internal region) AF2446a
    AF2446a (5 µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q9GZU7
Other Accession NP_067021.1, NP_872580.1, 58190
Reactivity Human, Mouse
Predicted Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 29203 Da
Additional Information
Gene ID 58190
Other Names Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1, 3.1.3.16, Nuclear LIM interactor-interacting factor 3, NLI-IF, NLI-interacting factor 3, Small C-terminal domain phosphatase 1, SCP1, Small CTD phosphatase 1, CTDSP1, NIF3, NLIIF, SCP1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTDSP1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTDSP1
Synonyms NIF3, NLIIF, SCP1
Function Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.
Cellular Location Nucleus. Note=Colocalizes with RNA polymerase II
Tissue Location Expression is restricted to non-neuronal tissues. Highest expression in skeletal muscle, spleen, lung and placenta
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognise both reported isoforms (NP_067021 and NP_872580)

References

Small CTD phosphatases function in silencing neuronal gene expression. Yeo M, Lee SK, Lee B, Ruiz EC, Pfaff SL, Gill GN. Science. 2005 Jan 28;307(5709):596-600. PMID: 15681389

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$ 341.00
Cat# AF2446a
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