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FEM1A / APRAP Antibody (C-Term)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9BSK4 |
| Other Accession | NP_061178.1, 55527 |
| Reactivity | Human |
| Predicted | Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/ml |
| Isotype | IgG |
| Calculated MW | 73639 Da |
| Gene ID | 55527 |
|---|---|
| Other Names | Protein fem-1 homolog A, FEM1a, FEM1-alpha, Prostaglandin E receptor 4-associated protein, FEM1A, EPRAP |
| Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | FEM1A / APRAP Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | FEM1A {ECO:0000303|PubMed:16254458, ECO:0000312|HGNC:HGNC:16934} |
|---|---|
| Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:29779948, PubMed:33398168, PubMed:33398170). The C- degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (PubMed:29779948, PubMed:33398168, PubMed:33398170). The CRL2(FEM1A) complex specifically recognizes proteins with an arginine at the C-terminus: recognizes and binds proteins ending with -Lys/Arg-Xaa-Arg and -Lys/Arg-Xaa-Xaa-Arg C- degrons, such as SIL1 or OR51B2, leading to their ubiquitination and degradation (PubMed:33398168, PubMed:33398170). Promotes ubiquitination and degradation of SLBP (PubMed:28118078). Involved in PGE2-EP4- mediated inhibition of inflammation of macrophages via interaction with NFKB1 and PTGER4 (By similarity). Promotes inflammation in brain microglia through MAP2K4/MKK4-mediated signaling (By similarity). |
| Cellular Location | Mitochondrion. Cytoplasm |
| Tissue Location | Present in macrophages derived from peripheral blood monocytes. Also present in atheromata (at protein level) |
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Provided below are standard protocols that you may find useful for product applications.
References
Prostaglandin E receptor type 4-associated protein interacts directly
with NF-kappaB1 and attenuates macrophage activation. Minami M, Shimizu K, Okamoto Y, Folco E, Ilasaca ML, Feinberg MW, Aikawa M, Libby P. J Biol Chem. 2008 Apr 11;283(15):9692-703. PMID: 18270204
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