Goat Anti-MAPRE3 Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q9UPY8 |
Other Accession | NP_036458, 22924, 100732 (mouse) |
Reactivity | Human, Mouse |
Predicted | Dog |
Host | Goat |
Clonality | Polyclonal |
Concentration | 100ug/200ul |
Isotype | IgG |
Calculated MW | 31982 Da |
Gene ID | 22924 |
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Other Names | Microtubule-associated protein RP/EB family member 3, EB1 protein family member 3, EBF3, End-binding protein 3, EB3, RP3, MAPRE3 |
Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Goat Anti-MAPRE3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MAPRE3 |
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Function | Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed:19255245, PubMed:28814570). Promotes microtubule growth (PubMed:19255245, PubMed:28814570). May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes (PubMed:19255245, PubMed:28814570). Also acts as a regulator of minus- end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570). |
Cellular Location | Cytoplasm, cytoskeleton. Note=Associated with the microtubule network. Detected at the plus end of microtubules |
Tissue Location | Predominantly expressed in brain and muscle. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene.
References
Molecular insights into mammalian end-binding protein heterodimerization. De Groot CO, et al. J Biol Chem, 2010 Feb 19. PMID 20008324.
Mitotic regulation of the stability of microtubule plus-end tracking protein EB3 by ubiquitin ligase SIAH-1 and Aurora mitotic kinases. Ban R, et al. J Biol Chem, 2009 Oct 9. PMID 19696028.
Mammalian end binding proteins control persistent microtubule growth. Komarova Y, et al. J Cell Biol, 2009 Mar 9. PMID 19255245.
p53 downstream target DDA3 is a novel microtubule-associated protein that interacts with end-binding protein EB3 and activates beta-catenin pathway. Hsieh PC, et al. Oncogene, 2007 Jul 26. PMID 17310996.
Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.
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