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Goat Anti-ZNF9 / CNBP Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-ZNF9 / CNBP Antibody AF2171a
    AF2171a (2 µg/ml) staining of Human Muscle lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P62633
Other Accession NP_003409, 7555
Reactivity Human
Predicted Mouse, Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 19463 Da
Additional Information
Gene ID 7555
Other Names Cellular nucleic acid-binding protein, CNBP, Zinc finger protein 9, CNBP, RNF163, ZNF9
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-ZNF9 / CNBP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CNBP (HGNC:13164)
Synonyms RNF163, ZNF9
Function Single-stranded DNA-binding protein that preferentially binds to the sterol regulatory element (SRE) sequence 5'-GTGCGGTG-3', and thereby mediates transcriptional repression (PubMed:2562787). Has a role as transactivator of the Myc promoter (By similarity). Binds single-stranded RNA in a sequence-specific manner (By similarity).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P53996}. Cytoplasm. Endoplasmic reticulum {ECO:0000250|UniProtKB:P53996} [Isoform 2]: Cytoplasm [Isoform 5]: Cytoplasm [Isoform 8]: Cytoplasm
Tissue Location Expressed in the liver, kidney, spleen, testis, lung, muscle and adrenal glands.
Research Areas
Citations (0)
citation

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Background

This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

References

Validation of sensitivity and specificity of tetraplet-primed PCR (TP-PCR) in the molecular diagnosis of myotonic dystrophy type 2 (DM2). Catalli C, et al. J Mol Diagn, 2010 Sep. PMID 20616365.
Myotonic dystrophy type 2 found in two of sixty-three persons diagnosed as having fibromyalgia. Auvinen S, et al. Arthritis Rheum, 2008 Nov. PMID 18975316.
Colocalization of ribonuclear inclusions with muscle blind like-proteins in a family with myotonic dystrophy type 2 associated with a short CCTG expansion. Lucchiari S, et al. J Neurol Sci, 2008 Dec 15. PMID 18804219.
Violating the splicing rules: TG dinucleotides function as alternative 3' splice sites in U2-dependent introns. Szafranski K, et al. Genome Biol, 2007. PMID 17672918.
Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.

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$ 341.00
Cat# AF2171a
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Availability: 7-10 days
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