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Goat Anti-SMO (internal) Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q99835 |
| Other Accession | NP_005622, 6608, 319757 (mouse), 25273 (rat) |
| Reactivity | Human |
| Predicted | Mouse, Rat, Zebrafish, Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 86397 Da |
| Gene ID | 6608 |
|---|---|
| Other Names | Smoothened homolog, SMO, Protein Gx, SMO, SMOH |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-SMO (internal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SMO |
|---|---|
| Synonyms | SMOH |
| Function | G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed:19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P56726}; Multi-pass membrane protein. Cell projection, cilium. Note=Cilium localization is promoted by SHH and is required for activity. {ECO:0000250|UniProtKB:P56726} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.
References
Immunohistochemical expression of SHH, PTC, SMO and GLI1 in glandular odontogenic cysts and dentigerous cysts. Zhang L, et al. Oral Dis, 2010 Jun 18. PMID 20561215.
Overexpression of smoothened activates the sonic hedgehog signaling pathway in pancreatic cancer-associated fibroblasts. Walter K, et al. Clin Cancer Res, 2010 Mar 15. PMID 20215540.
Smoothened as a new therapeutic target for human osteosarcoma. Hirotsu M, et al. Mol Cancer, 2010 Jan 12. PMID 20067614.
Hedgehog signaling maintains hair follicle stem cell phenotype in young and aged human skin. Ritti茅 L, et al. Aging Cell, 2009 Dec. PMID 20050020.
The variant rs1867277 in FOXE1 gene confers thyroid cancer susceptibility through the recruitment of USF1/USF2 transcription factors. Landa I, et al. PLoS Genet, 2009 Sep. PMID 19730683.
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