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Goat Anti-SAE1 / AOS1 Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9UBE0 |
| Other Accession | NP_005491, 10055 |
| Reactivity | Human |
| Predicted | Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 38450 Da |
| Gene ID | 10055 |
|---|---|
| Other Names | SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, SUMO-activating enzyme subunit 1, N-terminally processed, SAE1, AOS1, SUA1, UBLE1A |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-SAE1 / AOS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SAE1 |
|---|---|
| Synonyms | AOS1, SUA1, UBLE1A |
| Function | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
| Cellular Location | Nucleus. |
| Tissue Location | Expression level increases during S phase and drops in G2 phase (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).
References
Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931. A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers. Li T, et al. Arch Biochem Biophys, 2006 Sep 1. PMID 16620772. SUMO-1 controls the protein stability and the biological function of phosducin. Klenk C, et al. J Biol Chem, 2006 Mar 31. PMID 16421094. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560. Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.
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