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Goat Anti-PU.1 Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P17947 |
| Other Accession | NP_003111, 6688, 366126 (rat) |
| Reactivity | Human |
| Predicted | Rat |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 31083 Da |
| Gene ID | 6688 |
|---|---|
| Other Names | Transcription factor PU.1, 31 kDa-transforming protein, SPI1 |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-PU.1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPI1 |
|---|---|
| Function | Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed:33951726). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726). In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity). |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00237, ECO:0000269|PubMed:33951726} |
| Tissue Location | In the bone marrow, concentrated in hematopoietic stem cell, lymphoid progenitor, myeloid lineage (granulocyte macrophage progenitors, classical dendritic cells, monocytes) and B-cell clusters Among B-cells, predominantly expressed in pre-B1 cells (PubMed:33951726). Expressed in germinal center B-cells (PubMed:23166356). |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
References
PU.1 is regulated by NF-kappaB through a novel binding site in a 17 kb upstream enhancer element. Bonadies N, et al. Oncogene, 2010 Feb 18. PMID 19966852.
Heterozygous deletion of the PU.1 locus in human AML. Bonadies N, et al. Blood, 2010 Jan 14. PMID 19890096.
PU.1 regulates positive regulatory domain I-binding factor 1/Blimp-1 transcription in lymphoma cells. Desai S, et al. J Immunol, 2009 Nov 1. PMID 19828640.
PU.1 activation relieves GATA-1-mediated repression of Cebpa and Cbfb during leukemia differentiation. Burda P, et al. Mol Cancer Res, 2009 Oct. PMID 19825991.
PU.1 induces apoptosis in myeloma cells through direct transactivation of TRAIL. Ueno S, et al. Oncogene, 2009 Nov 19. PMID 19749795.
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