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Goat Anti-PEBP1 Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-PEBP1 Antibody AF1810b
    AF1810b staining (0.01 µg/ml) of A549 lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
    detail
  • IHC - Goat Anti-PEBP1 Antibody AF1810b
    AF1810b (1.5 µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P30086
Other Accession NP_002558, 5037, 23980 (mouse)
Reactivity Human, Rat
Predicted Mouse, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 21057 Da
Additional Information
Gene ID 5037
Other Names Phosphatidylethanolamine-binding protein 1, PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP, Hippocampal cholinergic neurostimulating peptide, HCNP, PEBP1, PBP, PEBP
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-PEBP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PEBP1
Synonyms PBP, PEBP
Function Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
citation

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References

Characterization of the Raf kinase inhibitory protein (RKIP) binding pocket: NMR-based screening identifies small-molecule ligands. Shemon AN, et al. PLoS One, 2010 May 5. PMID 20463977.
RKIP inhibits NF-kappaB in cancer cells by regulating upstream signaling components of the IkappaB kinase complex. Tang H, et al. FEBS Lett, 2010 Feb 19. PMID 20043910.
Reduction of Raf kinase inhibitor protein expression by Bcr-Abl contributes to chronic myelogenous leukemia proliferation. Takemura T, et al. J Biol Chem, 2010 Feb 26. PMID 20028985.
Raf kinase inhibitor protein enhances neuronal differentiation in human SH-SY5Y cells. Hellmann J, et al. Dev Neurosci, 2010 Mar. PMID 19955695.
Loss of RKIP expression is associated with poor survival in GISTs. Martinho O, et al. Virchows Arch, 2009 Sep. PMID 19705153.

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$ 341.00
Cat# AF1810b
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