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Goat Anti-PCK2 / PEPCK-M Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-PCK2 / PEPCK-M Antibody AF1797a
    AF1797a (2 µg/ml) staining of Human Liver lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • WB - Goat Anti-PCK2 / PEPCK-M Antibody AF1797a
    EB06944 (2µg/ml) staining of A431 (A), HEK293 (B) and (1ug/ml) HepG2 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
    detail
  • WB - Goat Anti-PCK2 / PEPCK-M Antibody AF1797a
    .EB06944 (2µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
    detail
  • IF - Goat Anti-PCK2 / PEPCK-M Antibody AF1797a
    EB06944 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
    detail
  • FC - Goat Anti-PCK2 / PEPCK-M Antibody AF1797a
    EB06944 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, Pep-ELISA
Primary Accession Q16822
Other Accession NP_004554, 5106
Reactivity Human, Mouse, Rat, Guinea Pig
Predicted Pig, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 70699 Da
Additional Information
Gene ID 5106
Other Names Phosphoenolpyruvate carboxykinase [GTP], mitochondrial, PEPCK-M, 4.1.1.32, PCK2, PEPCK2
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-PCK2 / PEPCK-M Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCK2 (HGNC:8725)
Synonyms PEPCK2
Function Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed:28955899). Can play an active role in glyceroneogenesis and gluconeogenesis (PubMed:28955899).
Cellular Location Mitochondrion.
Tissue Location Widely expressed..
Research Areas
Citations (0)
citation

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Background

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.

References

COMMON VARIANTS IN 40 GENES ASSESSED FOR DIABETES INCIDENCE AND RESPONSE TO METFORMIN AND LIFESTYLE INTERVENTIONS IN THE DIABETES PREVENTION PROGRAM. Jablonski KA, et al. Diabetes, 2010 Aug 3. PMID 20682687.
Fasting hyperglycemia is not associated with increased expression of PEPCK or G6Pc in patients with Type 2 Diabetes. Samuel VT, et al. Proc Natl Acad Sci U S A, 2009 Jul 21. PMID 19587243.
Endurance capacity, not body size, determines physical activity levels: role of skeletal muscle PEPCK. Novak CM, et al. PLoS One, 2009 Jun 12. PMID 19521512.
Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations. Lu Y, et al. J Lipid Res, 2008 Dec. PMID 18660489.
AMP-activated protein kinase regulates PEPCK gene expression by direct phosphorylation of a novel zinc finger transcription factor. Inoue E, et al. Biochem Biophys Res Commun, 2006 Dec 29. PMID 17097062.

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$ 341.00
Cat# AF1797a
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