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Goat Anti-NDUFS3 Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-NDUFS3 Antibody AF1713a
    AF1713a (0.3 µg/ml) staining of Human Frontal Cortex lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O75489
Other Accession NP_004542, 4722
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 30242 Da
Additional Information
Gene ID 4722
Other Names NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, 1.6.5.3, 1.6.99.3, Complex I-30kD, CI-30kD, NADH-ubiquinone oxidoreductase 30 kDa subunit, NDUFS3
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-NDUFS3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFS3
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:14729820, PubMed:30140060). Essential for the catalytic activity and assembly of complex I (PubMed:14729820, PubMed:24028823, PubMed:30140060).
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
Research Areas
Citations (0)
citation

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Background

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

References

Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease. Saada A, et al. Am J Hum Genet, 2009 Jun. PMID 19463981.
Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects. Saito A, et al. J Hum Genet, 2009 Jun. PMID 19343046.
Alterations in oligodendrocyte proteins, calcium homeostasis and new potential markers in schizophrenia anterior temporal lobe are revealed by shotgun proteome analysis. Martins-de-Souza D, et al. J Neural Transm, 2009 Mar. PMID 19034380.
Oxidative stress, telomere length and biomarkers of physical aging in a cohort aged 79 years from the 1932 Scottish Mental Survey. Starr JM, et al. Mech Ageing Dev, 2008 Dec. PMID 18977241.
Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death. Martinvalet D, et al. Cell, 2008 May 16. PMID 18485875.

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$ 341.00
Cat# AF1713a
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Availability: 7-10 days
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