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Goat Anti-GPX3 Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-GPX3 Antibody AF1500a
    AF1500a (1 µg/ml) staining of Mouse Heart lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - Goat Anti-GPX3 Antibody AF1500a
    AF1500a (20 µg/ml) staining of PFA-perfused cryosection of Porcin Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia
    detail
  • WB - Goat Anti-GPX3 Antibody AF1500a
    EB07685 (2µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
    detail
  • IHC - Goat Anti-GPX3 Antibody AF1500a
    EB07685 (4µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
    detail
  • IHC - Goat Anti-GPX3 Antibody AF1500a
    EB07685 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P22352
Other Accession NP_002075, 2878, 14778 (mouse), 64317 (rat)
Reactivity Mouse, Pig
Predicted Human, Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 25552 Da
Additional Information
Gene ID 2878
Other Names Glutathione peroxidase 3, GPx-3, GSHPx-3, 1.11.1.9, Extracellular glutathione peroxidase, Plasma glutathione peroxidase, GPx-P, GSHPx-P, GPX3, GPXP
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-GPX3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPX3 (HGNC:4555)
Synonyms GPXP
Function Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.
Cellular Location Secreted.
Tissue Location Secreted in plasma.
Research Areas
Citations (0)
citation

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Background

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

References

Assessing oxidative pathway genes as risk factors for bipolar disorder. Fullerton JM, et al. Bipolar Disord, 2010 Aug. PMID 20712757.
Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.
Functional glutathione peroxidase 3 polymorphisms associated with increased risk of Taiwanese patients with gastric cancer. Wang JY, et al. Clin Chim Acta, 2010 Oct 9. PMID 20576521.
Common polymorphisms in ITGA2, PON1 and THBS2 are associated with coronary atherosclerosis in a candidate gene association study of the Chinese Han population. Wang Y, et al. J Hum Genet, 2010 Aug. PMID 20485444.
Differential expression and distribution of placental glutathione peroxidases 1, 3 and 4 in normal and preeclamptic pregnancy. Mistry HD, et al. Placenta, 2010 May. PMID 20303587.

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$ 341.00
Cat# AF1500a
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