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Goat Anti-EBAG9 / RCAS1 Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-EBAG9 / RCAS1 Antibody AF1352a
    AF1352a (0.2 µg/ml) staining of A431 lysate (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O00559
Other Accession NP_004206, 9166, 55960 (mouse), 299864 (rat)
Reactivity Human
Predicted Mouse, Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 24377 Da
Additional Information
Gene ID 9166
Other Names Receptor-binding cancer antigen expressed on SiSo cells, Cancer-associated surface antigen RCAS1, Estrogen receptor-binding fragment-associated gene 9 protein, EBAG9, RCAS1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-EBAG9 / RCAS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EBAG9
Synonyms RCAS1
Function May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Cellular Location Golgi apparatus membrane; Single-pass type III membrane protein. Note=According to PubMed:10426319, it also exists as a soluble form which has the same biological activities The existence of such soluble form is however uncertain
Tissue Location Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs
Research Areas
Citations (0)
citation

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Background

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.

References

Analysis of the expression of human tumor antigens in ovarian cancer tissues. Ali-Fehmi R, et al. Cancer Biomark, 2010. PMID 20164540.
Receptor-binding cancer antigen expressed on SiSo cells induces apoptosis via ectodomain shedding. Sonoda K, et al. Exp Cell Res, 2010 Jul 1. PMID 20079734.
[Evaluation of RCAS1 as serum tumor marker for pancreatic cancer] Yang YC, et al. Zhonghua Wai Ke Za Zhi, 2009 Jul 1. PMID 19957811.
Expression of RCAS1 protein in microglia/macrophages accompanying brain tumours. An immunofluorescence study. Adamek D, et al. Folia Neuropathol, 2009. PMID 19813143.
Diagnostic and prognostic utility of serum receptor-binding cancer antigen expressed on SiSo cells (RCAS1) levels in colon cancer patients. Giaginis C, et al. Int J Biol Markers, 2009 Apr-Jun. PMID 19634109.

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$ 341.00
Cat# AF1352a
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