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Goat Anti-Cofilin 2 (muscle) Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-Cofilin 2 (muscle) Antibody AF1259a
    AF1259a (0.003 µg/ml) staining of Human Skeletal Muscle lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • WB - Goat Anti-Cofilin 2 (muscle) Antibody AF1259a
    AF1259a (0.001 µg/ml) staining of Rat Skeletal Muscle lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • WB - Goat Anti-Cofilin 2 (muscle) Antibody AF1259a
    HEK293 overexpressing Human Muscle Cofilin 2 (RC222215) and probed with AF1259a (mock transfection in first lane), tested by Origene.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9Y281
Other Accession NP_619579, 1073
Reactivity Human, Mouse, Rat
Predicted Pig, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 18737 Da
Additional Information
Gene ID 1073
Other Names Cofilin-2, Cofilin, muscle isoform, CFL2
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-Cofilin 2 (muscle) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CFL2
Function Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3 (PubMed:19752190). It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere (By similarity).
Cellular Location Nucleus matrix. Cytoplasm, cytoskeleton. Note=Colocalizes with CSPR3 in the Z line of sarcomeres.
Tissue Location Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues
Research Areas
Citations (0)
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Background

This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants.

References

Muscle LIM protein interacts with cofilin 2 and regulates F-actin dynamics in cardiac and skeletal muscle. Papalouka V, et al. Mol Cell Biol, 2009 Nov. PMID 19752190.
Cofilin activation in peripheral CD4 T cells of HIV-1 infected patients: a pilot study. Wu Y, et al. Retrovirology, 2008 Oct 17. PMID 18928553.
Nemaline myopathy with minicores caused by mutation of the CFL2 gene encoding the skeletal muscle actin-binding protein, cofilin-2. Agrawal PB, et al. Am J Hum Genet, 2007 Jan. PMID 17160903.
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.
Cofilin cross-bridges adjacent actin protomers and replaces part of the longitudinal F-actin interface. Kudryashov DS, et al. J Mol Biol, 2006 May 5. PMID 16530787.

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$ 341.00
Cat# AF1259a
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