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Goat Anti-APOA4 Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-APOA4 Antibody AF1074a
    AF1074a (0.01 µg/ml) staining of Human Duodenum lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - Goat Anti-APOA4 Antibody AF1074a
    AF1074a (2 µg/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P06727
Other Accession NP_000473, 337
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5mg/ml
Isotype IgG
Calculated MW 45372 Da
Additional Information
Gene ID 337
Other Names Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4, APOA4
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-APOA4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APOA4
Function May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Cellular Location Secreted.
Tissue Location Synthesized primarily in the intestine and secreted in plasma
Research Areas
Citations (0)
citation

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Background

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

References

The APOA1/C3/A4/A5 cluster and markers of allostatic load in the Boston Puerto Rican Health Study. Mattei J, et al. Nutr Metab Cardiovasc Dis, 2010 Jul 29. PMID 20674306. Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086. Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol? Rua帽o G, et al. Pharmacogenomics, 2010 Jul. PMID 20602615. No interaction between alcohol consumption and HDL-related genes on HDL cholesterol levels. Marques-Vidal P, et al. Atherosclerosis, 2010 Aug. PMID 20430392. APOA1 and APOA4 gene polymorphisms influence the effects of dietary fat on LDL particle size and oxidation in healthy young adults. Gomez P, et al. J Nutr, 2010 Apr. PMID 20164363.

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$ 341.00
Cat# AF1074a
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Availability: 7-10 days
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