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Phospho-Her3 (ErbB3)-Y1328 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Phospho-Her3 (ErbB3)-Y1328 Antibody AE1015b
    Western blot analysis of extracts from HUVEC cell using Her3 (ErbB3) Antibody (Y1328) (#AE1015a, Lane 1 and 2) and Phospho-Her3 (ErbB3)-Y1328 Antibody (#AE1015b, Lane 3 and 4).
    detail
  • IHC - Phospho-Her3 (ErbB3)-Y1328 Antibody AE1015b
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Her3 (ErbB3)-Y1328 Antibody (#AE1015b).
    detail
  • IF - Phospho-Her3 (ErbB3)-Y1328 Antibody AE1015b
    Immunofluorescence staining of methanol-fixed MCF7 cells using Phospho-Her3 (ErbB3)-Y1328 Antibody (#AE1015b, Red).
    detail
Western blot analysis of extracts from HUVEC cell using Her3 (ErbB3) Antibody (Y1328) (#AE1015a, Lane 1 and 2) and Phospho-Her3 (ErbB3)-Y1328 Antibody (#AE1015b, Lane 3 and 4).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession P21860
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Concentration 1mg/ml
Isotype Rabbit IgG
Calculated MW 148098 Da
Additional Information
Gene ID 2065
Other Names Receptor tyrosine-protein kinase erbB-3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3, ERBB3, HER3
Target/Specificity The antibody was affinity-purified from rabbit antiserum using epitope-specific phosphopeptide column, and the antibody against non-phosphopeptide was removed using non-phosphopeptide column corresponding to the phosphorylation site.
Dilution WB~~1:500~1:1000
IHC~~1:50~1:100
IF~~1:100~200
Format affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Her3 (ErbB3)-Y1328 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ERBB3
Synonyms HER3
Function Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). May also be activated by CSPG5 (PubMed:15358134). Involved in the regulation of myeloid cell differentiation (PubMed:27416908).
Cellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein
Tissue Location Epithelial tissues and brain.
Research Areas
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$ 355.00
Cat# AE1015b
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