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Functional Fas (human) Antibody, mAb (preservative free)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, IP |
|---|---|
| Primary Accession | P25445 |
| Reactivity | Human |
| Host | Purified From Concentrated Hybridoma Tissue Culture Supernatant. |
| Clonality | Monoclonal |
| Isotype | Mouse IgG3 |
| Gene Source | Human |
| Application Note | FC,IP,WB,Functional Application, Induces apoptosis with or without cross-linking (Protein A), depending on cell type. |
| Clone Names | APO-1-3 |
| Calculated MW | 37732 Da |
| Gene ID | 355 |
|---|---|
| Other Names | CD95; APO-1; TNFRSF6; Tumor Necrosis Factor Receptor Superfamily Member 6; Apoptosis-mediating Surface Antigen FAS |
| Target/Specificity | Recognizes human Fas. |
| Format | Liquid. In PBS. |
| Reconstitution & Storage | Stable for at least 1 year after receipt when stored at -20°C. |
| Precautions | Functional Fas (human) Antibody, mAb (preservative free) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | FAS |
|---|---|
| Synonyms | APT1, FAS1, TNFRSF6 |
| Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen- stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). |
| Cellular Location | [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Membrane raft [Isoform 3]: Secreted. [Isoform 5]: Secreted. |
| Tissue Location | Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Fas (CD95) is a member of the death receptor (DR) family, a subfamily of the tumor necrosis factor receptor superfamily. The formation of the Fas death-inducing signaling complex (DISC) is the initial step of Fas signaling. Activation of procaspase-8 at the DISC leads to the induction of DR-mediated apoptosis. Stimulation of Fas has been also reported to trigger non-apoptotic pathways. It has been shown that membrane-bound FasL is essential for the cytotoxic activity, whereas soluble FasL appears to promote autoimmunity and tumorigenesis via induction of non-apoptotic pathways, in particular NF-kappa.
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