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Anti-Phospho-VASP (S156) Rabbit Monoclonal Antibody

     
  •  - Anti-Phospho-VASP (S156) Rabbit Monoclonal Antibody ABO16772
    All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P50552
Host Rabbit
Isotype Rabbit IgG
Reactivity Human
Clonality Monoclonal
Format Liquid
Description Anti-Phospho-VASP (S156) Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human.
Additional Information
Gene ID 7408
Other Names Vasodilator-stimulated phosphoprotein, VASP, VASP
Calculated MW 39830 Da
Application Details WB 1:500-1:2000
IHC 1:50-1:200
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 32V43
Immunogen A synthesized peptide derived from human Phospho-VASP (S156)
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name VASP
Function Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Note=Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre- apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes)
Tissue Location Highly expressed in platelets.
Research Areas
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$ 370.00
Cat# ABO16772
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