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Anti-Phospho-Vimentin (S39) Rabbit Monoclonal Antibody

     
  •  - Anti-Phospho-Vimentin (S39) Rabbit Monoclonal Antibody ABO16664
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
    detail
  •  - Anti-Phospho-Vimentin (S39) Rabbit Monoclonal Antibody ABO16664
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
    detail
  •  - Anti-Phospho-Vimentin (S39) Rabbit Monoclonal Antibody ABO16664
    Western blot analysis of Phospho-Vimentin (S39) expression in HeLa treated with Calyculin A cell lysate.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P08670
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Phospho-Vimentin (S39) Rabbit Monoclonal Antibody . Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 7431
Other Names Vimentin, VIM
Calculated MW 53652 Da
Application Details WB 1:500-1:2000
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 31V35
Immunogen A synthesized peptide derived from human Phospho-Vimentin (S39)
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152}
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Research Areas
Citations (0)
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$ 370.00
Cat# ABO16664
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