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Anti-Phospho-Vimentin (S39) Rabbit Monoclonal Antibody

     
  •  - Anti-Phospho-Vimentin (S39) Rabbit Monoclonal Antibody ABO16664
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
    detail
  •  - Anti-Phospho-Vimentin (S39) Rabbit Monoclonal Antibody ABO16664
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
    detail
  •  - Anti-Phospho-Vimentin (S39) Rabbit Monoclonal Antibody ABO16664
    Western blot analysis of Phospho-Vimentin (S39) expression in HeLa treated with Calyculin A cell lysate.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P08670
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Phospho-Vimentin (S39) Rabbit Monoclonal Antibody . Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 7431
Other Names Vimentin, VIM
Calculated MW 53652 Da
Application Details WB 1:500-1:2000
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 31V35
Immunogen A synthesized peptide derived from human Phospho-Vimentin (S39)
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name VIM (HGNC:12692)
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152}
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Research Areas
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$ 370.00
Cat# ABO16664
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