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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Aconitase 2 Antibody Picoband™ (monoclonal, 4C12D1)   

Anti-Aconitase 2 Antibody Picoband™ (monoclonal, 4C12D1)

     
  •  - Anti-Aconitase 2 Antibody Picoband™ (monoclonal, 4C12D1) ABO16607
    Figure 1. Western blot analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human U-87MG whole cell lysates,
    Lane 3: human Jurkat whole cell lysates,
    Lane 4: human SH-SY5Y whole cell lysates,
    Lane 5: rat skeletal muscle tissue lysates,
    Lane 6: rat brain tissue lysates,
    Lane 7: mouse skeletal muscle tissue lysates,
    Lane 8: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Aconitase 2 antigen affinity purified monoclonal antibody (Catalog # M03096-3) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Aconitase 2 at approximately 85 kDa. The expected band size for Aconitase 2 is at 85 kDa.
    detail
  •  - Anti-Aconitase 2 Antibody Picoband™ (monoclonal, 4C12D1) ABO16607
    Figure 2. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3).
    Aconitase 2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
    detail
  •  - Anti-Aconitase 2 Antibody Picoband™ (monoclonal, 4C12D1) ABO16607
    Figure 3. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3).
    Aconitase 2 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
    detail
  •  - Anti-Aconitase 2 Antibody Picoband™ (monoclonal, 4C12D1) ABO16607
    Figure 4. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3).
    Aconitase 2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
    detail
  •  - Anti-Aconitase 2 Antibody Picoband™ (monoclonal, 4C12D1) ABO16607
    Figure 5. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3).
    Aconitase 2 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
    detail
  •  - Anti-Aconitase 2 Antibody Picoband™ (monoclonal, 4C12D1) ABO16607
    Figure 6. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3).
    Aconitase 2 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
    detail
  •  - Anti-Aconitase 2 Antibody Picoband™ (monoclonal, 4C12D1) ABO16607
    Figure 7. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3).
    Aconitase 2 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
    detail
  •  - Anti-Aconitase 2 Antibody Picoband™ (monoclonal, 4C12D1) ABO16607
    Figure 8. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3).
    Aconitase 2 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q99798
Host Mouse
Isotype Mouse IgG1
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Lyophilized
Description Anti-Aconitase 2 Antibody Picoband™ (monoclonal, 4C12D1) . Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Reconstitution Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Additional Information
Gene ID 50
Other Names Aconitate hydratase, mitochondrial, Aconitase, 4.2.1.3, Citrate hydro-lyase, ACO2
Calculated MW 85 kDa
Application Details Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat
Contents Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clone Names Clone: 4C12D1
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Aconitase 2, identical to the related mouse and rat sequences.
Purification Immunogen affinity purified.
Storage At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Protein Information
Name ACO2
Function Catalyzes the isomerization of citrate to isocitrate via cis- aconitate.
Cellular Location Mitochondrion {ECO:0000250|UniProtKB:P16276}.
Research Areas
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Background

Aconitase 2, mitochondrial is a protein that in humans is encoded by the ACO2 gene. The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

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$ 370.00
Cat# ABO16607
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