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Anti-ME1 Antibody Picoband™ (monoclonal, 5E5F7)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC |
|---|---|
| Primary Accession | P48163 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-ME1 Antibody Picoband™ (monoclonal, 5E5F7) . Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
| Reconstitution | Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml. |
| Gene ID | 4199 |
|---|---|
| Other Names | NADP-dependent malic enzyme, NADP-ME, 1.1.1.40, Malic enzyme 1, ME1 (HGNC:6983) |
| Calculated MW | 64 kDa |
| Application Details | Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Rat |
| Contents | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
| Clone Names | Clone: 5E5F7 |
| Immunogen | E.coli-derived human ME1 recombinant protein (Position: M1-Q572). |
| Purification | Immunogen affinity purified. |
| Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
| Name | ME1 (HGNC:6983) |
|---|---|
| Function | Catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP(+) and divalent metal ions, and decarboxylation of oxaloacetate. |
| Cellular Location | Cytoplasm. |
| Tissue Location | Expressed in all tissues tested including liver, placenta and white adipose tissue. |
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Provided below are standard protocols that you may find useful for product applications.
Background
NADP-dependent malic enzyme is a protein that in humans is encoded by the ME1 gene. This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
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