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Anti-Cyclin E1/CCNE1 Antibody Picoband™ (monoclonal, 8B9C3)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P24864 |
| Host | Mouse |
| Isotype | Mouse IgG2b |
| Reactivity | Human |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-Cyclin E1/CCNE1 Antibody Picoband™ (monoclonal, 8B9C3) . Tested in WB applications. This antibody reacts with Human. |
| Reconstitution | Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml. |
| Gene ID | 898 |
|---|---|
| Other Names | G1/S-specific cyclin-E1, CCNE1, CCNE |
| Calculated MW | 47 kDa |
| Application Details | Western blot, 0.25-0.5 µg/ml, Human |
| Contents | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
| Clone Names | Clone: 8B9C3 |
| Immunogen | E.coli-derived human Cyclin E1/CCNE1 recombinant protein (Position: R3-A386). |
| Purification | Immunogen affinity purified. |
| Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
| Name | CCNE1 |
|---|---|
| Synonyms | CCNE |
| Function | Essential for the control of the cell cycle at the G1/S (start) transition. |
| Cellular Location | Nucleus. |
| Tissue Location | Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. It is mapped to 19q12. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
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