Anti-CYB5R3 Rabbit Monoclonal Antibody

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18,000+ products cover the ten top research categories, including cancer, metabolism, cardiovascular, neuroscience, and stem cells. Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. Quality is embedded in the production process to assure the best antibody for you.

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Crown Products

Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications.

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New Products

Abgent develops 1000+ new products per year. We provide tools for path-breaking research by developing antibodies that detect a comprehensive library of novel and established targets. For established targets we seek to add antibodies that recognize new epitopes, including post-translational modifications such as phosphorylation and methylation. For new targets we consult with leading experts to accelerate development of antibodies that will propel state-of-the-art research in cellular health and disease.

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Current Promotion

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Research Focus

Greneral Collections

Peptides

SARS Peptides

Individual peptides for SARS-CoV-2 Spike glycoprotein. In order to study the specificity of cellular immune responses against SARS CoV-2 and potential immunity caused by other human Corona Viruses, Abcepta provides Spike peptide individually, as pools and in plate. These peptides can be used for antigen specific T-cell stimulation in T-cell assays or T-cell expansion.

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Synthetic Peptides

All peptides are manufactured in the San Diego, California. Custom peptide services include long peptides (>100 aa), cyclic peptides, difficult sequences, fluorescent labels, phospho-peptides and other post-translational modifications.

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Blocking Peptides

The majority of Abcepta antibodies are produced using peptide immunogens. These immuno-specific peptides can be used as blocking agents when using the complementary antibodies in a range of applications.

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Cell/Tissues/Lysates

Abcepta's portfolio of cell lines, tissues and lysates are drawn from a range of species and immortalized cell lines. All our cell lines and lysates are validated in key applications.

These tissue lysates can be used in applications such as SDS-PAGE and Western blotting. Whole cell lysates can be used as positive controls for applications such as ELISAs, immunoprecipitation (IP) and Western blotting. Obtaining high-quality whole cell lysates can be tedious as well as time consuming. Abcepta offers a comprehensive portfolio of whole cell lysates for research use. Browse our catalog to find the right whole cell lysate for your experiment.

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Citations

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

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Submit your citation using an Abcepta antibody to
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Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, IHC
Primary Accession	P00387
Host	Rabbit
Isotype	IgG
Reactivity	Rat, Human
Clonality	Monoclonal
Format	Liquid
Description	Anti-CYB5R3 Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human, Rat.

Gene ID	1727
Other Names	NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, 1.6.2.2, Diaphorase-1, CYB5R3 (HGNC:2873), DIA1
Calculated MW	34 kDa
Application Details	WB 1:500-1:2000 IHC 1:50-1:200
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: 28C75
Immunogen	A synthesized peptide derived from human CYB5R3
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Name	CYB5R3 (HGNC:2873)
Synonyms	DIA1
Function	Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
Cellular Location	[Isoform 1]: Endoplasmic reticulum membrane; Lipid-anchor {ECO:0000250\|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250\|UniProtKB:P20070}. Mitochondrion outer membrane; Lipid-anchor {ECO:0000250\|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250\|UniProtKB:P20070}
Tissue Location	[Isoform 2]: Expressed at late stages of erythroid maturation.

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