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Anti-P2Y12 Rabbit Monoclonal Antibody

     
  •  - Anti-P2Y12 Rabbit Monoclonal Antibody ABO16281
    Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
    detail
  •  - Anti-P2Y12 Rabbit Monoclonal Antibody ABO16281
    Figure 1. Western blot analysis of P2RY12 using anti-P2RY12 antibody (M01136).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Jurkat whole cell lysates,
    Lane 2: human SH-SY5Y whole cell lysates,
    Lane 3: human U-87 MG whole cell lysates,
    Lane 4: human U251 whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: rat C6 whole cell lysates,
    Lane 7: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2RY12 antigen affinity purified monoclonal antibody (Catalog # M01136) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2RY12 at approximately 50 kDa. The expected band size for P2RY12 is at 39 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IP
Primary Accession Q9H244
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-P2Y12 Rabbit Monoclonal Antibody . Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 64805
Other Names P2Y purinoceptor 12, P2Y12, ADP-glucose receptor, ADPG-R, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, P2Y(ADP), SP1999, P2RY12, HORK3
Calculated MW 50 kDa
Application Details WB 1:500-1:2000
IP 1:50
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 28P17
Immunogen A synthesized peptide derived from human P2Y12
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name P2RY12
Synonyms HORK3
Function Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation.
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Highly expressed in the platelets, lower levels in the brain. Lowest levels in the lung, appendix, pituitary and adrenal gland. Expressed in the spinal cord and in the fetal brain
Research Areas
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$ 370.00
Cat# ABO16281
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