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> home > Products > Primary Antibodies > Signal Transduction > Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody
Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody
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Application 
| WB, IP |
|---|---|
| Primary Accession | Q9D2Y4 |
| Host | Rabbit |
| Isotype | IgG |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Format | Liquid |
| Description | Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody . Tested in WB, IP applications. This antibody reacts with Mouse. |
| Gene ID | 74568 |
|---|---|
| Other Names | Mixed lineage kinase domain-like protein, Mlkl {ECO:0000303|PubMed:23835476, ECO:0000312|MGI:MGI:1921818} |
| Calculated MW | 54 kDa |
| Application Details | WB 1:500-1:2000 IP 1:50 |
| Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| Clone Names | Clone: 27M64 |
| Immunogen | A synthesized peptide derived from human Phospho-MLKL (S345) |
| Purification | Affinity-chromatography |
| Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Name | Mlkl {ECO:0000303|PubMed:23835476, ECO:0000312|MGI:MGI:1921818} |
|---|---|
| Function | Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:23835476, PubMed:24012422, PubMed:24019532, PubMed:27321907, PubMed:32200799, PubMed:32296175). Does not have protein kinase activity (PubMed:24012422). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:23835476, PubMed:24012422, PubMed:24019532, PubMed:27321907). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (PubMed:32200799, PubMed:32296175). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (By similarity). |
| Cellular Location | Cytoplasm. Cell membrane. Nucleus. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (By similarity). Localizes to the nucleus in response to orthomyxoviruses infection (PubMed:32200799). {ECO:0000250|UniProtKB:Q8NB16, ECO:0000269|PubMed:32200799} |
| Tissue Location | Highly expressed in thymus, colon, intestine, liver, spleen and lung. Expressed at much lower level in skeletal muscle, heart and kidney. Not detected in brain |
Research Areas
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$ 370.00
Cat# ABO16185
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