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Anti-Collagen III Rabbit Monoclonal Antibody

     
  •  - Anti-Collagen III Rabbit Monoclonal Antibody ABO16095
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
    detail
  •  - Anti-Collagen III Rabbit Monoclonal Antibody ABO16095
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
    detail
  •  - Anti-Collagen III Rabbit Monoclonal Antibody ABO16095
    All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
    detail
  •  - Anti-Collagen III Rabbit Monoclonal Antibody ABO16095
    Western blot analysis of Collagen III expression in (1) A431 cell lysate; (2) MCF7 cell lysate.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, ICC, IP, FC
Primary Accession P02461
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Collagen III Rabbit Monoclonal Antibody . Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 1281
Other Names Collagen alpha-1(III) chain, COL3A1
Calculated MW 150 kDa-200 kDa
Application Details WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 26C74
Immunogen A synthesized peptide derived from Collagen III
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name COL3A1
Function Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
Cellular Location Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}
Research Areas
Citations (0)
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$ 370.00
Cat# ABO16095
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