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Anti-Phospho-PDHA1 (S293) Rabbit Monoclonal Antibody

     
  •  - Anti-Phospho-PDHA1 (S293) Rabbit Monoclonal Antibody ABO15955
    Figure 1. Western blot analysis of PDHA1 using anti-PDHA1 antibody (P01906).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human 293T whole cell lysates,
    Lane 2: human A549 whole cell lysates,
    Lane 3: human Hela whole cell lysates,
    Lane 4: human HepG2 whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: rat kidney tissue lysates,
    Lane 7: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PDHA1 antigen affinity purified monoclonal antibody (Catalog # P01906) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDHA1 at approximately 43 kDa. The expected band size for PDHA1 is at 43 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP
Primary Accession P08559
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Phospho-PDHA1 (S293) Rabbit Monoclonal Antibody . Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 5160
Other Names Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, 1.2.4.1, PDHE1-A type I, PDHA1, PHE1A
Calculated MW 43 kDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 25P34
Immunogen A synthesized peptide derived from human Phospho-PDHA1 (S293)
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name PDHA1
Synonyms PHE1A
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Cellular Location Mitochondrion matrix.
Tissue Location Ubiquitous.
Research Areas
Citations (0)
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$ 370.00
Cat# ABO15955
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