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Anti-CRKII Rabbit Monoclonal Antibody

     
  •  - Anti-CRKII Rabbit Monoclonal Antibody ABO15876
    Western blot analysis of CRKII expression in K562 cell lysate.
    detail
  •  - Anti-CRKII Rabbit Monoclonal Antibody ABO15876
    IF analysis of CRKII using anti-CRKII antibody (M02533-3) and anti-Beta Tubulin antibody (M01857-3).
    CRKII was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-CRKII Antibody (M02533-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, ICC, IP, FC
Primary Accession P46108
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-CRKII Rabbit Monoclonal Antibody . Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 1398
Other Names Adapter molecule crk, Proto-oncogene c-Crk, p38, CRK
Calculated MW 38 kDa
Application Details WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:100
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 24C53
Immunogen A synthesized peptide derived from human CRKII
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name CRK
Function Involved in cell branching and adhesion mediated by BCAR1- CRK-RAPGEF1 signaling and activation of RAP1.
Cellular Location Cytoplasm. Cell membrane. Note=Translocated to the plasma membrane upon cell adhesion.
Research Areas
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$ 154.00
Cat# ABO15876
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