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Anti-FGF19 Rabbit Monoclonal Antibody

     
  •  - Anti-FGF19 Rabbit Monoclonal Antibody ABO15845
    Figure 1. Western blot analysis of FGF19 using anti-FGF19 antibody (M01191).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human SH-SY5Y whole cell lysates,
    Lane 2: human U-87MG whole cell lysates,
    Lane 3: human CACO-2 whole cell lysates,
    Lane 4: human HepG2 whole cell lysates,
    Lane 5: rat liver tissue lysates,
    Lane 6: mouse liver tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF19 antigen affinity purified monoclonal antibody (Catalog # M01191) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF19 at approximately 24 kDa. The expected band size for FGF19 is at 24 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O95750
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-FGF19 Rabbit Monoclonal Antibody . Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 9965
Other Names Fibroblast growth factor 19, FGF-19, FGF19
Calculated MW 36 kDa
Application Details WB 1:500-1:2000
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 24F22
Immunogen A synthesized peptide derived from human FGF19
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name FGF19
Function Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4.
Cellular Location Secreted.
Tissue Location Expressed in fetal brain, cartilage, retina, and adult gall bladder.
Research Areas
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$ 370.00
Cat# ABO15845
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