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Anti-Engrailed 1 Rabbit Monoclonal Antibody

     
  •  - Anti-Engrailed 1 Rabbit Monoclonal Antibody ABO15803
    Western blot analysis of Engrailed 1 expression in 293T cell lysate.
    detail
  •  - Anti-Engrailed 1 Rabbit Monoclonal Antibody ABO15803
    Figure 1. Western blot analysis of EN1 using anti-EN1 antibody (M05395).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat brain tissue lysates,
    Lane 2: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EN1 antigen affinity purified monoclonal antibody (Catalog # M05395) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EN1 at approximately 55 kDa. The expected band size for EN1 is at 40 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IP, FC
Primary Accession Q05925
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Engrailed 1 Rabbit Monoclonal Antibody . Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 2019
Other Names Homeobox protein engrailed-1, Homeobox protein en-1, Hu-En-1, EN1
Calculated MW 55 kDa
Application Details WB 1:500-1:2000
IP 1:50
FC 1:100
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 23E80
Immunogen A synthesized peptide derived from human Engrailed 1
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name EN1
Function Required for proper formation of the apical ectodermal ridge and correct dorsal-ventral patterning in the limb.
Cellular Location Nucleus.
Research Areas
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$ 370.00
Cat# ABO15803
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