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Anti-Peroxiredoxin 5 Rabbit Monoclonal Antibody

     
  •  - Anti-Peroxiredoxin 5 Rabbit Monoclonal Antibody ABO15699
    Figure 1. Western blot analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody (M02891-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human HepG2 whole cell lysates,
    Lane 2: human Hela whole cell lysates,
    Lane 3: human PC-3 whole cell lysates,
    Lane 4: human MCF-7 whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: rat lung tissue lysates,
    Lane 7: mouse brain tissue lysates,
    Lane 8: mouse lung tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 5 antigen affinity purified monoclonal antibody (Catalog # M02891-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxiredoxin 5 at approximately 17 kDa. The expected band size for Peroxiredoxin 5 is at 22 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, FC
Primary Accession P30044
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Peroxiredoxin 5 Rabbit Monoclonal Antibody . Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 25824
Other Names Peroxiredoxin-5, mitochondrial, 1.11.1.24, Alu corepressor 1, Antioxidant enzyme B166, AOEB166, Liver tissue 2D-page spot 71B, PLP, Peroxiredoxin V, Prx-V, Peroxisomal antioxidant enzyme, TPx type VI, Thioredoxin peroxidase PMP20, Thioredoxin-dependent peroxiredoxin 5, PRDX5 (HGNC:9355), ACR1
Calculated MW 17 kDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:80
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 22P75
Immunogen A synthesized peptide derived from human Peroxiredoxin 5
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name PRDX5 (HGNC:9355)
Synonyms ACR1
Function Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.
Cellular Location [Isoform Mitochondrial]: Mitochondrion
Tissue Location Widely expressed..
Research Areas
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$ 370.00
Cat# ABO15699
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