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Anti-Oxytocin Receptor Rabbit Monoclonal Antibody

     
  •  - Anti-Oxytocin Receptor Rabbit Monoclonal Antibody ABO15661
    Figure 1. Western blot analysis of OXTR using anti-OXTR antibody (M01566).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Jurkat whole cell lysates,
    Lane 2: human PC-3 whole cell lysates,
    Lane 3: human A549 whole cell lysates,
    Lane 4: human Caco-2 whole cell lysates,
    Lane 5: rat PC-12 whole cell lysates,
    Lane 6: mouse RAW264.7 whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXTR antigen affinity purified monoclonal antibody (Catalog # M01566) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXTR at approximately 43 kDa. The expected band size for OXTR is at 43 kDa.
    detail
  •  - Anti-Oxytocin Receptor Rabbit Monoclonal Antibody ABO15661
    Figure 2. IHC analysis of OXTR using anti-OXTR antibody (M01566).
    OXTR was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-OXTR Antibody (M01566) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
  •  - Anti-Oxytocin Receptor Rabbit Monoclonal Antibody ABO15661
    Figure 3. IHC analysis of OXTR using anti-OXTR antibody (M01566).
    OXTR was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-OXTR Antibody (M01566) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P30559
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Oxytocin Receptor Rabbit Monoclonal Antibody . Tested in WB, IHC application. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 5021
Other Names Oxytocin receptor, OT-R, OXTR
Calculated MW 43 kDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 22O37
Immunogen A synthesized peptide derived from human Oxytocin Receptor
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name OXTR
Function Receptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.
Cellular Location Cell membrane; Multi-pass membrane protein.
Research Areas
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$ 370.00
Cat# ABO15661
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