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Anti-DDB2 Rabbit Monoclonal Antibody
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Application 
| WB, IHC, IF, ICC, FC |
|---|---|
| Primary Accession | Q92466 |
| Host | Rabbit |
| Isotype | IgG |
| Reactivity | Human |
| Clonality | Monoclonal |
| Format | Liquid |
| Description | Anti-DDB2 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human. |
| Gene ID | 1643 |
|---|---|
| Other Names | DNA damage-binding protein 2, DDB p48 subunit, DDBb, Damage-specific DNA-binding protein 2, UV-damaged DNA-binding protein 2, UV-DDB 2, DDB2 |
| Calculated MW | 45 kDa |
| Application Details | WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 FC 1:20 |
| Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| Clone Names | Clone: 20D73 |
| Immunogen | A synthesized peptide derived from human DDB2 |
| Purification | Affinity-chromatography |
| Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Name | DDB2 |
|---|---|
| Function | Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (PubMed:10882109, PubMed:11278856, PubMed:11705987, PubMed:12732143, PubMed:15882621, PubMed:16473935, PubMed:18593899, PubMed:32789493, PubMed:9892649). Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair (PubMed:10882109, PubMed:11278856, PubMed:11705987, PubMed:12944386, PubMed:14751237, PubMed:16260596, PubMed:32789493). The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches (PubMed:10882109, PubMed:11278856, PubMed:11705987, PubMed:12944386, PubMed:16260596). Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4- DDB-RBX1) (PubMed:12732143, PubMed:15882621, PubMed:16473935, PubMed:18593899, PubMed:26572825). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV- induced DNA damage (PubMed:16473935, PubMed:16678110). The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair (PubMed:16473935, PubMed:16678110). The DDB2-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER (PubMed:15882621). The DDB2-CUL4-ROC1 complex also ubiquitinates KAT7/HBO1 in response to DNA damage, leading to its degradation: recognizes KAT7/HBO1 following phosphorylation by ATR (PubMed:26572825). |
| Cellular Location | Nucleus. Chromosome. Note=Accumulates at sites of DNA damage following UV irradiation. |
| Tissue Location | Ubiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed. |
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Application Protocols
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$ 370.00
Cat# ABO15497
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