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Anti-Lumican Rabbit Monoclonal Antibody

     
  •  - Anti-Lumican Rabbit Monoclonal Antibody ABO15484
    Western blot analysis of Lumican expression in Jurkat cell lysate.
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  •  - Anti-Lumican Rabbit Monoclonal Antibody ABO15484
    Figure 2. IF analysis of Lumican using anti-Lumican antibody (M01034-1).
    Lumican was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 rabbit anti-Lumican Antibody (M01034-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
    detail
  •  - Anti-Lumican Rabbit Monoclonal Antibody ABO15484
    All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
    detail
  •  - Anti-Lumican Rabbit Monoclonal Antibody ABO15484
    All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
    detail
  •  - Anti-Lumican Rabbit Monoclonal Antibody ABO15484
    All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, FC
Primary Accession P51884
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Lumican Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 4060
Other Names Lumican, Keratan sulfate proteoglycan lumican, KSPG lumican, LUM, LDC, SLRR2D
Calculated MW 38-60 kDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 20L60
Immunogen A synthesized peptide derived from human Lumican
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name LUM
Synonyms LDC, SLRR2D
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Cornea and other tissues.
Research Areas
Citations (0)
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$ 370.00
Cat# ABO15484
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