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Anti-eIF2B epsilon Rabbit Monoclonal Antibody

     
  •  - Anti-eIF2B epsilon Rabbit Monoclonal Antibody ABO15309
    Western blot analysis of eIF2B epsilon expression in Hela cell lysate.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC
Primary Accession Q13144
Host Rabbit
Isotype IgG
Reactivity Human
Clonality Monoclonal
Format Liquid
Description Anti-eIF2B epsilon Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Additional Information
Gene ID 8893
Other Names Translation initiation factor eIF2B subunit epsilon, eIF2B GDP-GTP exchange factor subunit epsilon, EIF2B5, EIF2BE
Calculated MW 80 kDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 18E85
Immunogen A synthesized peptide derived from human eIF2B epsilon
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name EIF2B5
Synonyms EIF2BE
Function Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit (PubMed:25858979, PubMed:27023709, PubMed:31048492). Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl- initiator methionine tRNA available to the ribosome and consequently global translation is repressed (PubMed:25858979, PubMed:31048492).
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:P56287}
Research Areas
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$ 370.00
Cat# ABO15309
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