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Anti-beta Tubulin Mouse Monoclonal Antibody

     
  •  - Anti-beta Tubulin Mouse Monoclonal Antibody ABO15138
    Western blot analysis of beta Actin expression in (1) A431 cell lysate; (2) Rat brain lysate; (3) Mouse kidney lysate; (4) Chicken lung lysate; (5) Hamster spleen lysate with beta Tubulin Mouse Monoclonal Antibody.
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  •  - Anti-beta Tubulin Mouse Monoclonal Antibody ABO15138
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using beta Tubulin Mouse Monoclonal Antibody.
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  •  - Anti-beta Tubulin Mouse Monoclonal Antibody ABO15138
    Immunofluorescent analysis of Hela cells, using beta Tubulin mAb.
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  •  - Anti-beta Tubulin Mouse Monoclonal Antibody ABO15138
    Figure 1. Western blot analysis of beta Tubulin using anti-beta Tubulin antibody (M05613-5).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human K562 whole cell lysates,
    Lane 3: human MCF-7 whole cell lysates,
    Lane 4: human A549 whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: rat kidney tissue lysates,
    Lane 7: mouse brain tissue lysates,
    Lane 8: mouse kidney tissue lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-beta Tubulin antigen affinity purified monoclonal antibody (Catalog # M05613-5) at 1:3000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta Tubulin at approximately 50 kDa. The expected band size for beta Tubulin is at 50 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC
Primary Accession P07437
Host Mouse
Isotype IgG1
Reactivity Rat, Human, Mouse, Hamster, Chicken
Clonality Monoclonal
Format Liquid
Description Anti-beta Tubulin Mouse Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat, Chicken, Hamster.
Additional Information
Gene ID 203068
Other Names Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5
Calculated MW 55 kDa
Application Details WB 1:3000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Contents Mouse IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 17T13
Immunogen A synthesized peptide derived from human beta Tubulin
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name TUBB
Synonyms TUBB5
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Cellular Location Cytoplasm, cytoskeleton
Tissue Location Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Research Areas
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$ 154.00
Cat# ABO15138
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