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Anti-Neurofibromin/NF1 Antibody Picoband™ (monoclonal, 4C6F10)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, FC |
|---|---|
| Primary Accession | P21359 |
| Host | Mouse |
| Isotype | Mouse IgG2b |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-Neurofibromin/NF1 Antibody Picoband™ (monoclonal, 4C6F10) . Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
| Reconstitution | Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml. |
| Gene ID | 4763 |
|---|---|
| Other Names | Neurofibromin, Neurofibromatosis-related protein NF-1, Neurofibromin truncated, NF1 |
| Calculated MW | 319 kDa |
| Application Details | Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat Flow Cytometry, 1-3 µg/1x10^6 cells, Human |
| Contents | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
| Clone Names | Clone: 4C6F10 |
| Immunogen | E.coli-derived human Neurofibromin/NF1 recombinant protein (Position: R160-Q270). |
| Purification | Immunogen affinity purified. |
| Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
| Name | NF1 |
|---|---|
| Function | Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity. |
| Cellular Location | Nucleus. Nucleus, nucleolus. Cell membrane |
| Tissue Location | Detected in brain, peripheral nerve, lung, colon and muscle. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Neurofibromin 1 (NF1) is a gene in humans that is located on chromosome 17. This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene.
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