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Anti-Semaphorin 3B/SEMA3B Antibody Picoband™ (monoclonal, 9C4F7)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, ICC, FC |
|---|---|
| Primary Accession | Q13214 |
| Host | Mouse |
| Isotype | Mouse IgG2b |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-Semaphorin 3B/SEMA3B Antibody Picoband™ (monoclonal, 9C4F7) . Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
| Reconstitution | Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml. |
| Gene ID | 7869 |
|---|---|
| Other Names | Semaphorin-3B, Sema A(V), Semaphorin-V, Sema V, SEMA3B, SEMA5, SEMAA |
| Calculated MW | 83 kDa |
| Application Details | Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Flow Cytometry, 1-3 µg/1x10^6 cells, Human |
| Contents | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
| Clone Names | Clone: 9C4F7 |
| Immunogen | E.coli-derived human Semaphorin 3B/SEMA3B recombinant protein (Position: Y55-R624). |
| Purification | Immunogen affinity purified. |
| Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
| Name | SEMA3B |
|---|---|
| Synonyms | SEMA5, SEMAA |
| Function | Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. |
| Cellular Location | Secreted. Endoplasmic reticulum. Note=Accumulates in the endoplasmic reticulum |
| Tissue Location | Expressed abundantly but differentially in a variety of neural and nonneural tissues |
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Provided below are standard protocols that you may find useful for product applications.
Background
Semaphorin-3Bis aproteinthat in humans is encoded by theSEMA3Bgene. It is mapped to 3p21.31. The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.
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