Anti-CD20/MS4A1 Antibody Picoband™ (monoclonal, 4I11)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF |
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Primary Accession | P11836 |
Host | Mouse |
Isotype | Mouse IgG2a |
Reactivity | Human |
Clonality | Monoclonal |
Format | Lyophilized |
Description | Anti-CD20/MS4A1 Antibody Picoband™ (monoclonal, 4I11) . Tested in IF, WB applications. This antibody reacts with Human. |
Reconstitution | Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml. |
Gene ID | 931 |
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Other Names | B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20 |
Calculated MW | 37 kDa |
Application Details | Western blot, 0.25-0.5 µg/ml, Human Immunofluorescence, 5 µg/ml, Human |
Contents | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
Clone Names | Clone: 4I11 |
Immunogen | E.coli-derived human CD20/MS4A1 recombinant protein (Position: M1-P297). |
Purification | Immunogen affinity purified. |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Name | MS4A1 |
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Synonyms | CD20 |
Function | B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739). |
Cellular Location | Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor. Note=Constitutively associated with membrane rafts. |
Tissue Location | Expressed on B-cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What’s more, this protein may be involved in the regulation of B-cell activation and proliferation.
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