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Anti-DDX4/MVH Antibody Picoband™ (monoclonal, 3C3)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF |
|---|---|
| Primary Accession | Q9NQI0 |
| Host | Mouse |
| Isotype | Mouse IgG2b |
| Reactivity | Rat, Mouse |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-DDX4/MVH Antibody Picoband™ (monoclonal, 3C3) . Tested in IF, IHC, WB applications. This antibody reacts with Mouse, Rat. |
| Reconstitution | Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml. |
| Gene ID | 54514 |
|---|---|
| Other Names | Probable ATP-dependent RNA helicase DDX4, 3.6.4.13, DEAD box protein 4, Vasa homolog, DDX4, VASA |
| Calculated MW | 79 kDa |
| Application Details | Western blot, 0.25-0.5 µg/ml, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Mouse, Rat Immunofluorescence, 5 µg/ml, Mouse, Rat |
| Contents | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
| Clone Names | Clone: 3C3 |
| Immunogen | E.coli-derived human DDX4/MVH recombinant protein (Position: D3-D666). |
| Purification | Immunogen affinity purified. |
| Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
| Name | DDX4 |
|---|---|
| Synonyms | VASA |
| Function | ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity). Required for PIWIL2 slicing- triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q61496}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:Q61496} Note=Component of the meiotic nuage, also named P granule, a germ-cell- specific organelle required to repress transposon activity during meiosis. {ECO:0000250|UniProtKB:Q61496} |
| Tissue Location | Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. |
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Provided below are standard protocols that you may find useful for product applications.
Background
DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
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