Anti-POR Antibody Picoband™ (monoclonal, 7F5)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, FC |
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Primary Accession | P16435 |
Host | Mouse |
Isotype | Mouse IgG2b |
Reactivity | Human |
Clonality | Monoclonal |
Format | Lyophilized |
Description | Anti-POR Antibody Picoband™ (monoclonal, 7F5) . Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. |
Reconstitution | Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml. |
Gene ID | 5447 |
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Other Names | NADPH--cytochrome P450 reductase {ECO:0000255|HAMAP-Rule:MF_03212}, CPR {ECO:0000255|HAMAP-Rule:MF_03212}, P450R {ECO:0000255|HAMAP-Rule:MF_03212}, 1.6.2.4 {ECO:0000255|HAMAP-Rule:MF_03212}, POR {ECO:0000255|HAMAP-Rule:MF_03212}, CYPOR |
Calculated MW | 77 kDa |
Application Details | Western blot, 0.25-0.5 µg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human Flow Cytometry, 1-3 µg/1x10^6 cells, Human |
Contents | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
Clone Names | Clone: 7F5 |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human POR, different from the related mouse and rat sequences by five amino acids. |
Purification | Immunogen affinity purified. |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Name | POR {ECO:0000255|HAMAP-Rule:MF_03212} |
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Synonyms | CYPOR |
Function | This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. |
Cellular Location | Endoplasmic reticulum membrane {ECO:0000255|HAMAP-Rule:MF_03212}; Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03212}; Cytoplasmic side {ECO:0000255|HAMAP- Rule:MF_03212} |
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Provided below are standard protocols that you may find useful for product applications.
Background
POR is a membrane-boundenzyme required for electron transfer from NADPH to cytochrome P450 in the endoplasmic reticulum of theeukaryotic cell. The gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.
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