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Anti-Alpha A Crystallin Antibody Picoband™ (monoclonal, 10B9)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, ICC |
|---|---|
| Primary Accession | P02489 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-Alpha A Crystallin Antibody Picoband™ (monoclonal, 10B9) . Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
| Reconstitution | Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml. |
| Gene ID | 102724652;1409 |
|---|---|
| Other Names | Alpha-crystallin A chain, Heat shock protein beta-4, HspB4, Heat shock protein family B member 4, Alpha-crystallin A(1-172), Alpha-crystallin A(1-168), Alpha-crystallin A(1-162), CRYAA, CRYA1, HSPB4 |
| Calculated MW | 20-23 kDa |
| Application Details | Western blot, 0.25-0.5 µg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human |
| Contents | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
| Clone Names | Clone: 10B9 |
| Immunogen | E. coli-derived human Alpha A Crystallin recombinant protein (Position: M1-S173). Human Alpha A Crystallin shares 94.8% amino acid (aa) sequence identity with both mouse and rat Alpha A Crystallin. |
| Purification | Immunogen affinity purified. |
| Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
| Name | CRYAA |
|---|---|
| Synonyms | CRYA1, HSPB4 |
| Function | Contributes to the transparency and refractive index of the lens (PubMed:18302245). In its oxidized form (absence of intramolecular disulfide bond), acts as a chaperone, preventing aggregation of various proteins under a wide range of stress conditions (PubMed:18199971, PubMed:19595763, PubMed:22120592, PubMed:31792453). Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA (PubMed:28935373). |
| Cellular Location | Cytoplasm. Nucleus. Note=Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles |
| Tissue Location | Expressed in the eye lens (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).
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