Anti-REA/PHB2 Antibody Picoband™ (monoclonal, 2B5)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, FC |
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Primary Accession | Q99623 |
Host | Mouse |
Isotype | Mouse IgG2b |
Reactivity | Rat, Human, Mouse |
Clonality | Monoclonal |
Format | Lyophilized |
Description | Anti-REA/PHB2 Antibody Picoband™ (monoclonal, 2B5) . Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 11331 |
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Other Names | Prohibitin-2, B-cell receptor-associated protein BAP37, D-prohibitin, Repressor of estrogen receptor activity, PHB2 {ECO:0000312|EMBL:AAH14766.1, ECO:0000312|HGNC:HGNC:30306} |
Calculated MW | 32 kDa |
Application Details | Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat Flow Cytometry, 1-3 µg/1x10^6 cells, Human |
Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4. |
Clone Names | Clone: 2B5 |
Immunogen | E.coli-derived human REA/PHB2 recombinant protein (Position: M1-K299). |
Purification | Immunogen affinity purified. |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Name | PHB2 {ECO:0000312|EMBL:AAH14766.1, ECO:0000312|HGNC:HGNC:30306} |
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Function | Protein with pleiotropic attributes mediated in a cell- compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors and sex steroid hormones in the nucleus. |
Cellular Location | Mitochondrion inner membrane. Cytoplasm. Nucleus. Cell membrane Note=Localizes within both nucleus and cytoplasm in proliferative primary myoblasts and mostly within the nucleus of differentiated primary myoblasts. [Isoform 2]: Mitochondrion inner membrane |
Tissue Location | Expressed in myoblasts. |
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Provided below are standard protocols that you may find useful for product applications.
Background
PHB2 (Prohibitin 2), also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al. (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta (ESR2), REA suppressed activation of a reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.
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