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Anti- IL3 Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P01586 |
| Host | Rat |
| Isotype | Rat IgG1 |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti- IL3 Monoclonal Antibody . Tested in WB applications. This antibody reacts with Mouse. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500 µg/ml. |
| Gene ID | 16187 |
|---|---|
| Other Names | Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor, Il3, Csfmu, Il-3 |
| Calculated MW | 19 kDa |
| Application Details | Western blot, 0.25-0.5 µg/ml, Mouse |
| Contents | PBS, pH 7.0. Contains no stabilizers or preservatives |
| Clone Names | Clone: AFIB-9 |
| Immunogen | COS-expressed, recombinant mouse IL-3 |
| Purification | Immunogen affinity purified. |
| Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Name | Il3 |
|---|---|
| Synonyms | Csfmu, Il-3 |
| Function | Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage- restricted cells. Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (By similarity). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program (PubMed:10376805, PubMed:31990690, PubMed:8378315). Alternatively, contributes to cell survival under oxidative stress in non- hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (By similarity). |
| Cellular Location | Secreted. |
| Tissue Location | Activated T-cells, mast cells, natural killer cells |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.
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